1RI4

Structure and mechanism of mRNA cap (guanine N-7) methyltransferase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 31 2 1
Unit cell lengths	63.803, 63.803, 112.683
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 * - 2.400
R-factor	0.215
Rwork	0.215
R-free	0.28000 *
Structure solution method	SAD+MIR
RMSD bond length	0.008
RMSD bond angle	1.150 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.500
High resolution limit [Å]	2.400	2.400
Rmerge	0.066	0.231 *
Total number of observations	124773 *
Number of reflections	9398
<I/σ(I)>	26.4
Completeness [%]	86.5	64.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	8 *	291	1.2M Na/K tartrate, 50mM BIS/TRIS, 20mM DTT, pH 6.25, VAPOR DIFFUSION, HANGING DROP, temperature 291K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	6.5 (mg/ml)
2	1	drop		220 (mM)
3	1	drop	Tris-HCl	20 (mM)	pH8.0
4	1	drop	beta-mercaptoethanol	1 (mM)
5	1	reservoir	sodium/pootassium tartrate	1.2 (M)
6	1	reservoir	bis-tris	50 (mM)	pH6.0
7	1	reservoir	dithiothreitol	20 (mM)