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1RHI

HUMAN RHINOVIRUS 3 COAT PROTEIN

Experimental procedure
Source typeSYNCHROTRON
Source detailsCHESS BEAMLINE F1
Synchrotron siteCHESS
BeamlineF1
Temperature [K]293
Detector technologyIMAGE PLATE
Collection date1993-09-16
DetectorFUJI
Spacegroup nameP 21 2 21
Unit cell lengths397.900, 341.800, 301.700
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution5.000 - 3.000
R-factor0.281

*

Rwork0.284
R-free0.28800
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)HUMAN RHINOVIRUS 14
RMSD bond length0.009

*

RMSD bond angle1.800

*

Data reduction softwareMGROSC
Data scaling softwareMGRSCL
Phasing softwareX-PLOR (3.1)
Refinement softwareX-PLOR (3.1)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]30.0003.120
High resolution limit [Å]2.8002.800
Rmerge0.1200.189
Number of reflections730925139901

*

<I/σ(I)>33
Completeness [%]73.147
Redundancy2.61.6
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP7.2RESERVOIR SOLUTION: 10MM CACL2 AND 0.75% PEG8000 IN A 0.25M HEPES/0.75M NACL/PH7.2 BUFFER HANGING DROP: 5UL OF 10MG/ML VIRUS + 5UL OF RESERVOIR SOLUTION, vapor diffusion - hanging drop
Crystallization Reagents in Literatures
IDcrystal IDsolutionreagent nameconcentration (unit)details
11dropvirus5 (mg/ml)
21drop5 (mM)
31dropPEG80000.375 (%)
41dropHEPES0.125 (M)
51drop0.375 (M)
61reservoir10 (mM)
71reservoirPEG80000.75 (%)
81reservoirHEPES0.25 (M)
91reservoir0.75 (M)

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