1RHI
HUMAN RHINOVIRUS 3 COAT PROTEIN
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1993-09-16 |
| Detector | FUJI |
| Spacegroup name | P 21 2 21 |
| Unit cell lengths | 397.900, 341.800, 301.700 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 5.000 - 3.000 |
| R-factor | 0.281 * |
| Rwork | 0.284 |
| R-free | 0.28800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | HUMAN RHINOVIRUS 14 |
| RMSD bond length | 0.009 * |
| RMSD bond angle | 1.800 * |
| Data reduction software | MGROSC |
| Data scaling software | MGRSCL |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.120 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.120 | 0.189 |
| Number of reflections | 730925 | 139901 * |
| <I/σ(I)> | 3 | 3 |
| Completeness [%] | 73.1 | 47 |
| Redundancy | 2.6 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | RESERVOIR SOLUTION: 10MM CACL2 AND 0.75% PEG8000 IN A 0.25M HEPES/0.75M NACL/PH7.2 BUFFER HANGING DROP: 5UL OF 10MG/ML VIRUS + 5UL OF RESERVOIR SOLUTION, vapor diffusion - hanging drop |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | virus | 5 (mg/ml) | |
| 2 | 1 | drop | 5 (mM) | ||
| 3 | 1 | drop | PEG8000 | 0.375 (%) | |
| 4 | 1 | drop | HEPES | 0.125 (M) | |
| 5 | 1 | drop | 0.375 (M) | ||
| 6 | 1 | reservoir | 10 (mM) | ||
| 7 | 1 | reservoir | PEG8000 | 0.75 (%) | |
| 8 | 1 | reservoir | HEPES | 0.25 (M) | |
| 9 | 1 | reservoir | 0.75 (M) |
