1RH2

RECOMBINANT HUMAN INTERFERON-ALPHA 2B

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	103
Detector technology	IMAGE PLATE
Collection date	1995-12
Detector	RIGAKU
Spacegroup name	P 1 21 1
Unit cell lengths	62.400, 75.500, 148.200
Unit cell angles	90.00, 90.80, 90.00

Refinement procedure

Resolution	6.000 - 2.900
R-factor	0.227
Rwork	0.227
R-free	0.31100
Structure solution method	MULTIPLE ISOMORPHOUS REPLACEMENT
RMSD bond length	0.016
RMSD bond angle	21.400 *
Data reduction software	MOLECULAR (STRUCTURE CORP.)
Data scaling software	MSC
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	6.000	3.000
High resolution limit [Å]	2.900	2.900
Rmerge	0.095	0.240
Total number of observations	217393 *
Number of reflections	31925
<I/σ(I)>	10	3.2
Completeness [%]	96.0	92
Redundancy	6.8	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.6	23 *	PROTEIN WAS CRYSTALLIZED FROM 40MM ZINC ACETATE, 30MM CACODYLATE, PH 5.6; MACRO SEEDING WAS PERFORMED TO GET REASONABLE SIZE CRYSTALS., macroseeding

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	40 (mg/ml)
2	1	reservoir		40 (mM)
3	1	reservoir	cacodylate	30 (mM)