1RDA
CRYSTAL STRUCTURES OF RIBONUCLEASE HI ACTIVE SITE MUTANTS FROM ESCHERICHIA COLI
Experimental procedure
Spacegroup name | P 21 21 21 |
Unit cell lengths | 44.170, 87.230, 35.270 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 2.150 |
R-factor | 0.189 |
RMSD bond length | 0.015 |
RMSD bond angle | 0.035 |
Refinement software | PROLSQ |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 9 * | 20 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 7.5 (mg/ml) | |
2 | 1 | drop | Tris-HCl | 50 (mM) | |
3 | 1 | reservoir | Tris-HCl | 200 (mM) |