1RD8
Crystal Structure of the 1918 Human H1 Hemagglutinin Precursor (HA0)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-02-21 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.984 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 190.529, 109.953, 136.238 |
Unit cell angles | 90.00, 108.63, 90.00 |
Refinement procedure
Resolution | 49.400 * - 3.000 |
R-factor | 0.27128 |
Rwork | 0.270 |
R-free | 0.29600 * |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.019 |
RMSD bond angle | 1.850 * |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.400 * | 3.070 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.146 * | 0.520 * |
Total number of observations | 125868 * | |
Number of reflections | 51346 | 3368 * |
<I/σ(I)> | 7.6 | 1.4 |
Completeness [%] | 96.4 | 95.1 * |
Redundancy | 2.5 | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 294 | sodium phosphate, potassium phosphate, sodium citrate, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 15 (mg/ml) | |
2 | 1 | reservoir | sodium dihydrogen phosphate | 1.68 (M) | |
3 | 1 | reservoir | dipotassium hydrogen phosphate | 0.32 (M) | |
4 | 1 | reservoir | phosphate citrate | 0.1 (M) | pH5.5 |