1RBZ
Human GAR Tfase complex structure with polyglutamated 10-(trifluoroacetyl)-5,10-dideazaacyclic-5,6,7,8-tetrahydrofolic acid
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-06-14 |
Detector | ADSC QUANTUM 9 |
Wavelength(s) | 0.992 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 126.157, 126.157, 93.987 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.000 - 2.100 |
R-factor | 0.246 |
Rwork | 0.245 |
R-free | 0.27000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1njs |
RMSD bond length | 0.009 |
RMSD bond angle | 1.490 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.000 | 2.130 |
High resolution limit [Å] | 2.060 | 2.060 |
Number of reflections | 53508 | |
<I/σ(I)> | 24.9 | 1.71 |
Completeness [%] | 99.3 | 96.8 |
Redundancy | 4.15 | 3.68 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4 | 277 | PEG 1500, Sodium Acetate, pH 4., VAPOR DIFFUSION, SITTING DROP, temperature 277K |