1RAM
A NOVEL DNA RECOGNITION MODE BY NF-KB P65 HOMODIMER
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 105 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-10-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 65.690, 81.080, 167.560 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.000 - 2.700 |
| R-factor | 0.222 * |
| Rwork | 0.222 |
| R-free | 0.32400 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 26.500 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.843) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 2.700 |
| Rmerge | 0.036 |
| Total number of observations | 71540 * |
| Number of reflections | 24346 |
| Completeness [%] | 97.0 |
| Redundancy | 2.94 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 18 * | crystallized from PEG 3350, CACL2, BETA-OCTYL GLUCOPYRANOSIDE, HEPES and GLYCEROL, pH 7.50, VAPOR DIFFUSION, HANGING DROP, temperature 291.00K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PRECIPITATING SOLUTION | ||
| 2 | 1 | 2 | PEG 3350 | ||
| 3 | 1 | 2 | CACL2 | ||
| 4 | 1 | 2 | DTT | ||
| 5 | 1 | 2 | BETA-OCTYL GLUCOPYRANOSIDE | ||
| 6 | 1 | 2 | HEPES | ||
| 7 | 1 | 3 | PRECIPITATING SOLUTION | ||
| 8 | 1 | 3 | GLYCEROL |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | DNA complex | 7.5 (mg/ml) | |
| 10 | 1 | reservoir | beta-octyl glucopyranoside | 0.1 (%) | |
| 11 | 1 | reservoir | HEPES | 50 (mM) | |
| 2 | 1 | drop | PEG3350 | 4 (%) | |
| 3 | 1 | drop | 50 (mM) | ||
| 4 | 1 | drop | dithiothreitol | 5 (mM) | |
| 5 | 1 | drop | beta-octyl glucopyranoside | 0.05 (%) | |
| 6 | 1 | drop | 25 (mM) | ||
| 7 | 1 | reservoir | PEG3350 | 8 (%) | |
| 8 | 1 | reservoir | 100 (mM) | ||
| 9 | 1 | reservoir | dithiothreitol | 10 (mM) |
