1R9N
Crystal Structure of human dipeptidyl peptidase IV in complex with a decapeptide (tNPY) at 2.3 Ang. Resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.3 |
Synchrotron site | ALS |
Beamline | 5.0.3 |
Temperature [K] | 173 |
Detector technology | CCD |
Collection date | 2003-03-15 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.01 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 122.606, 122.701, 145.405 |
Unit cell angles | 90.00, 114.88, 90.00 |
Refinement procedure
Resolution | 41.170 - 2.300 |
R-factor | 0.2127 |
Rwork | 0.211 |
R-free | 0.25140 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | : 1R9M |
RMSD bond length | 0.010 |
RMSD bond angle | 1.276 |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | AMoRE |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 23270.000 | 2.360 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.075 | 0.429 |
Number of reflections | 161260 | |
<I/σ(I)> | 11.7 | 1.8 |
Completeness [%] | 94.2 | |
Redundancy | 2 | 1.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.25 | 298 | PEG 2000, Bicine, pH 8.25, VAPOR DIFFUSION, SITTING DROP, temperature 298K |