1R9M
Crystal Structure of Human Dipeptidyl Peptidase IV at 2.1 Ang. Resolution.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.3 |
| Synchrotron site | ALS |
| Beamline | 5.0.3 |
| Temperature [K] | 173 |
| Detector technology | CCD |
| Collection date | 2002-09-15 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.01 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 121.823, 124.056, 144.491 |
| Unit cell angles | 90.00, 114.71, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.100 |
| R-factor | 0.21906 |
| Rwork | 0.218 |
| R-free | 0.24883 |
| Structure solution method | MIR |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.457 |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.424 | |
| Number of reflections | 218087 | |
| <I/σ(I)> | 19.9 | 2.3 |
| Completeness [%] | 76.8 | |
| Redundancy | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | Tris-HCl, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
