1R9C
Crystal Structure of Fosfomycin Resistance Protein FosX from Mesorhizobium Loti
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-09-19 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 45.034, 84.024, 66.861 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.830 |
| R-factor | 0.2046 |
| Rwork | 0.202 |
| R-free | 0.24300 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lqp |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.363 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.900 |
| High resolution limit [Å] | 1.830 | 1.830 |
| Number of reflections | 21017 | |
| <I/σ(I)> | 17.9 | 1.9 |
| Completeness [%] | 91.1 | 55.8 |
| Redundancy | 3.8 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 8.5 | 20 * | PEG8000, Tris, Li2SO4, MnCl2, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | PEG8000 | 32 (%) | |
| 2 | 1 | drop | Tris | 100 (mM) | pH8.5 |
| 3 | 1 | drop | 50 (mM) | ||
| 4 | 1 | reservoir | 1 (mM) |
