1R8Y
Crystal Structure of Mouse Glycine N-Methyltransferase (Monoclinic Form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR591 |
| Temperature [K] | 103 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-06-09 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 107.980, 108.300, 119.010 |
| Unit cell angles | 90.00, 93.71, 90.00 |
Refinement procedure
| Resolution | 13.000 - 3.000 |
| R-factor | 0.224 |
| Rwork | 0.222 |
| R-free | 0.28508 |
| Structure solution method | MIR |
| Starting model (for MR) | 1xva |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.650 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 40592 | |
| <I/σ(I)> | 19.6 | 3.05 |
| Completeness [%] | 99.2 | 100 |
| Redundancy | 3.6 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.2 | 298 | PEG4000, isopropanol, Tris, pH 8.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
