1R8I

Crystal structure of TraC

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2001-08-24
Detector	CUSTOM-MADE
Wavelength(s)	0.97931
Spacegroup name	H 3 2
Unit cell lengths	144.015, 144.015, 91.880
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	25.000 * - 3.000
R-factor	0.263
Rwork	0.263
R-free	0.28830 *
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.380 *
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	3.110
High resolution limit [Å]	3.000	3.000
Rmerge	0.068 *	0.301 *
Total number of observations	106523 *
Number of reflections	13072
<I/σ(I)>	21.4	3.2
Completeness [%]	92.1	92.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.25	293	ammonium sulfate, sodium citrate, sodium chloride, glycerol, pH 6.25, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	1.26-1.34 (M)
2	1	reservoir	sodium cirtate	100 (mM)	pH6.25
3	1	reservoir		0.2 (M)
4	1	reservoir	glycerol	5 (%)