1R8H

Comparison of the structure and DNA binding properties of the E2 proteins from an oncogenic and a non-oncogenic human papillomavirus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX14.1
Synchrotron site	SRS
Beamline	PX14.1
Temperature [K]	100
Detector technology	CCD
Collection date	2001-10-20
Detector	ADSC QUANTUM 4
Wavelength(s)	1.488
Spacegroup name	P 61
Unit cell lengths	71.664, 71.664, 195.004
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 1.900
R-factor	0.1947
Rwork	0.191
R-free	0.26280 *
Structure solution method	MIR
RMSD bond length	0.019 *
RMSD bond angle	1.691 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000 *	1.970
High resolution limit [Å]	1.900	1.900
Rmerge	0.044 *	0.221 *
Number of reflections	42743
Completeness [%]	93.2 *	58.8
Redundancy	3.0 *	1.4 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	Ammonium sulfate, sodium chloride, sodium hepes, beta-mercaptoethanol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	2.5 (mg/ml)
2	1	drop	beta-mercaptoethanol	0.1 (%)
3	1	drop	sodium azide	0.02 (%(w/v))
4	1	reservoir	ammonium sulfate	1-2 (M)
5	1	reservoir		0.1 (M)
6	1	reservoir	HEPES	0.1 (M)	pH7.5