1R7H
NrdH-redoxin of Corynebacterium ammoniagenes forms a domain-swapped dimer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-09-14 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 63 |
| Unit cell lengths | 89.630, 89.630, 40.150 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 23.700 - 2.690 |
| R-factor | 0.24513 |
| Rwork | 0.243 |
| R-free | 0.28760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1h75 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.894 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.19) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.760 |
| High resolution limit [Å] | 2.690 | 2.690 |
| Number of reflections | 5187 | |
| Completeness [%] | 98.6 | 98.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.86 | 293 | Na-citrate, Tris, MPD, spermidine, pH 7.86, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
