1R6Z
The Crystal Structure of the Argonaute2 PAZ domain (as a MBP fusion)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X26C |
| Synchrotron site | NSLS |
| Beamline | X26C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 65 |
| Unit cell lengths | 89.811, 89.811, 380.326 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 * - 2.801* |
| Rwork | 0.227 |
| R-free | 0.28090 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ANF(no sugar no waters) |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.320 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.105 * | 0.548 * |
| Total number of observations | 172206 * | |
| Number of reflections | 41500 | 3383 * |
| <I/σ(I)> | 12.74 | 1.756 |
| Completeness [%] | 97.3 | 78.9 |
| Redundancy | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 17 * | PEG2KMME, NiCl2, TrisHCl, NaF, meso-erythritol, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG2000 MME | 15 (%(w/v)) | |
| 2 | 1 | reservoir | 10 (mM) | ||
| 3 | 1 | reservoir | ethylene glycol | 5 (%(v/v)) | |
| 4 | 1 | reservoir | 100 (mM) | ||
| 5 | 1 | reservoir | meso-erythritol | 30 (mM) | |
| 6 | 1 | reservoir | Tris-HCl | 100 (mM) | pH8.5 |
| 7 | 1 | drop | protein | 15 (mg/ml) | |
| 8 | 1 | drop | Tris-HCl | 100 (mM) | pH8.0 |
| 9 | 1 | drop | 100 (mM) |
