1R68
Role of the amino sugar in DNA binding of disaccharide anthracyclines: crystal structure of MAR70/d(CGATCG) complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 298 |
Detector technology | DIFFRACTOMETER |
Collection date | 2003-02-15 |
Detector | RIGAKU AFC-5R |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 28.190, 28.190, 53.250 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 1.200 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.017 |
Data reduction software | TEXAN |
Data scaling software | TEXSAN |
Phasing software | AMoRE |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 1.300 |
High resolution limit [Å] | 1.200 | 1.200 |
Rmerge | 0.114 | |
Number of reflections | 6369 | |
<I/σ(I)> | 4.3 | |
Completeness [%] | 97.8 | 87 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6 | 298 | Magnesium chloride, cacodylate, spermine, MPD, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K, pH 6.00 |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | Magnesium chloride | ||
10 | 1 | 2 | H2O | ||
2 | 1 | 1 | cacodylate | ||
3 | 1 | 1 | spermine | ||
4 | 1 | 1 | MPD | ||
5 | 1 | 1 | H2O | ||
6 | 1 | 2 | Magnesium chloride | ||
7 | 1 | 2 | cacodylate | ||
8 | 1 | 2 | spermine | ||
9 | 1 | 2 | MPD |