1R5U
RNA POLYMERASE II TFIIB COMPLEX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-01-23 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 212.438, 217.182, 422.876 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 4.500 |
| R-factor | 0.345 |
| Rwork | 0.345 |
| R-free | 0.37300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1i6h |
| RMSD bond length | 0.024 |
| RMSD bond angle | 1.689 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 4.700 |
| High resolution limit [Å] | 4.500 | 4.500 |
| Rmerge | 0.091 * | 0.277 * |
| Number of reflections | 57906 | 8951 * |
| <I/σ(I)> | 14 | 3.4 |
| Completeness [%] | 99.4 | 99.2 |
| Redundancy | 4.4 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 6.5 * | 298 | A.L., Gnatt, (2001) Science, 292, 1876. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | dioxane | 50 (mM) | |
| 2 | 1 | reservoir | dithiothreitol | 10 (mM) | |
| 3 | 1 | reservoir | ammonium phosphate | 390 (mM) | |
| 4 | 1 | reservoir | PEG6000 |
