1R4U

URATE OXIDASE FROM ASPERGILLUS FLAVUS COMPLEXED WITH ITS INHIBITOR OXONIC ACID

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LURE BEAMLINE DW32
Synchrotron site	LURE
Beamline	DW32
Temperature [K]	283
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Wavelength(s)	0.972
Spacegroup name	I 2 2 2
Unit cell lengths	79.702, 96.075, 105.589
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.650
Rwork	0.157
R-free	0.18200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1UOX
RMSD bond length	0.012
RMSD bond angle	1.331
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	BUSTER-TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.690
High resolution limit [Å]	1.650	1.650
Rmerge	0.064	0.375
Number of reflections	48706
<I/σ(I)>	13	2.9
Completeness [%]	99.1	95.9
Redundancy	8.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	291	8.5MG/ML PROTEIN, 0.2MG/ML OXONIC ACID, 5-7% W/V PEG 8000, 100MM TRIS/HCL, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 291K