1R4P
Shiga toxin type 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-04-20 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 61 |
| Unit cell lengths | 143.960, 143.960, 59.300 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.100 - 1.770 |
| R-factor | 0.153 |
| Rwork | 0.151 |
| R-free | 0.18370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.017 |
| RMSD bond angle | 2.000 |
| Data scaling software | SCALEPACK |
| Phasing software | CCP4 ((ALMN) |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.100 | 1.880 |
| High resolution limit [Å] | 1.770 | 1.770 |
| Number of reflections | 66890 | |
| Completeness [%] | 97.6 | 95 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 294 | 4 M sodium formate, 0.1 M MES, 50 mM 3-(1-pyridino)-1-propanesulfonate, 1% ethylene glycol, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
