1R4I
Crystal Structure of Androgen Receptor DNA-Binding Domain Bound to a Direct Repeat Response Element
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-07-09 |
Detector | MARRESEARCH |
Wavelength(s) | 1.0000, 1.2831 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 137.890, 137.890, 85.710 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 3.100 |
Rwork | 0.246 |
R-free | 0.26400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | ER Complex from PDB entry 1HCQ |
RMSD bond length | 0.008 |
RMSD bond angle | 1.300 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.210 |
High resolution limit [Å] | 3.100 | 3.100 |
Rmerge | 0.096 | 0.620 |
Number of reflections | 17313 | |
<I/σ(I)> | 20.4 | 2.5 |
Completeness [%] | 99.7 | 99.1 |
Redundancy | 10.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | PEG 400, magnesium chloride, Tris, DTT, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG 400 | ||
2 | 1 | 1 | magnesium chloride | ||
3 | 1 | 1 | Tris | ||
4 | 1 | 1 | DTT | ||
5 | 1 | 1 | H2O | ||
6 | 1 | 2 | PEG 400 | ||
7 | 1 | 2 | magnesium chloride | ||
8 | 1 | 2 | DTT | ||
9 | 1 | 2 | H2O |