1R4C
N-Truncated Human Cystatin C; Dimeric Form With 3D Domain Swapping
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-03-24 |
| Detector | MARRESEARCH |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 97.147, 99.639, 206.066 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.180 |
| R-factor | 0.22 |
| Rwork | 0.218 |
| R-free | 0.25900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | HUMAN CYSTATIN C DIMER WITH SWAPPED DOMAINS (PDB ENTRY 1G96) |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.635 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.260 |
| High resolution limit [Å] | 2.180 | 2.180 |
| Rmerge | 0.041 | 0.126 |
| Number of reflections | 52404 | |
| <I/σ(I)> | 33.6 | 10.9 |
| Completeness [%] | 99.2 | 98.8 |
| Redundancy | 9.3 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.1 | 292 | 0.4M (NH4)H2PO4, VAPOR DIFFUSION, HANGING DROP, temperature 292K, pH 8.1 |
