1R3J
potassium channel KcsA-Fab complex in high concentration of Tl+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-01-19 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9504 |
| Spacegroup name | I 4 |
| Unit cell lengths | 154.740, 154.740, 76.380 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.660 - 1.900 |
| R-factor | 0.21 |
| Rwork | 0.208 |
| R-free | 0.22800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1k4c |
| RMSD bond length | 0.006 |
| RMSD bond angle | 25.700 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.069 | 0.482 |
| Number of reflections | 69035 | |
| <I/σ(I)> | 15.2 | 2.1 |
| Completeness [%] | 97.1 | 93 |
| Redundancy | 3.1 | 2.52 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 * | 20 * | PEG400, sodium acetate, magnesium acetate, pH 5.4, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris | 50 (mM) | pH7.5 |
| 2 | 1 | drop | DM | 5 (mM) | |
| 3 | 1 | drop | 160 (mM) | ||
| 4 | 1 | reservoir | PEG400 | 18-25 (%(w/v)) | |
| 5 | 1 | reservoir | magnesium acetate | 50 (mM) | |
| 6 | 1 | reservoir | sodium acetate | 50 (mM) | pH5.4 |
| 7 | 1 | drop | 80 (mM) |
