1R35

MURINE INDUCIBLE NITRIC OXIDE SYNTHASE OXYGENASE DIMER, TETRAHYDROBIOPTERIN AND 4R-FLUORO-N6-ETHANIMIDOYL-L-LYSINE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-BM
Synchrotron site	APS
Beamline	17-BM
Temperature [K]	125
Detector technology	CCD
Collection date	2000-03-09
Detector	MARRESEARCH
Wavelength(s)	1.0
Spacegroup name	P 61 2 2
Unit cell lengths	214.280, 214.280, 117.130
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	8.000 - 2.300
R-factor	0.252
Rwork	0.252
R-free	0.28500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	murine iNOS holoenzyme structure PDB ID 2NOD
RMSD bond length	0.008
RMSD bond angle	1.400
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR (98.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.071	0.649
Number of reflections	67757
<I/σ(I)>		2.1
Completeness [%]		75.6
Redundancy	11.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1