1R2Q

Crystal Structure of Human Rab5a GTPase Domain at 1.05 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	100
Detector technology	CCD
Collection date	2002-07-17
Detector	ADSC QUANTUM 4
Wavelength(s)	0.916
Spacegroup name	P 21 21 21
Unit cell lengths	35.727, 64.573, 66.180
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.020 - 1.050
R-factor	0.124
Rwork	0.124
R-free	0.16880
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	1n6n
RMSD bond length	0.015
RMSD bond angle	0.036
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.020	1.070
High resolution limit [Å]	1.050	1.050
Rmerge	0.065	0.520
Number of reflections	68904
<I/σ(I)>	18.7	2.2
Completeness [%]	95.2	79
Redundancy	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	277	PEG 6000, SODIUM CHLORIDE, MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K