1R2B

Crystal structure of the BCL6 BTB domain complexed with a SMRT co-repressor peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2001-11-20
Detector	MARRESEARCH
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	54.228, 38.543, 76.659
Unit cell angles	90.00, 92.92, 90.00

Refinement procedure

Resolution	30.000 * - 2.200
R-factor	0.227
Rwork	0.227
R-free	0.26770 *
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.400
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.050 *	0.256 *
Number of reflections	16405
<I/σ(I)>	24	3.7
Completeness [%]	99.2	92.1
Redundancy	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	PEG 3350, sodium acetate, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	reservoir	PEG3350	25 (%)
3	1	reservoir	ammonium acetate	0.2 (M)