1R1H

STRUCTURAL ANALYSIS OF NEPRILYSIN WITH VARIOUS SPECIFIC AND POTENT INHIBITORS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ENRAF-NONIUS FR591
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2001-08-09
Detector	MARRESEARCH
Wavelength(s)	1.54
Spacegroup name	P 32 2 1
Unit cell lengths	107.791, 107.791, 113.090
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 1.950
R-factor	0.21143
Rwork	0.209
R-free	0.26024
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.856
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.070
High resolution limit [Å]	1.950	1.950
Rmerge	0.062	0.463
Number of reflections	53437
<I/σ(I)>	26.2	2.5
Completeness [%]	95.9	93.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1