1R0L
1-deoxy-D-xylulose 5-phosphate reductoisomerase from zymomonas mobilis in complex with NADPH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2002-02-10 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.076 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 87.700, 93.200, 98.600 |
| Unit cell angles | 90.00, 90.50, 90.00 |
Refinement procedure
| Resolution | 29.630 - 2.700 |
| R-factor | 0.249 |
| Rwork | 0.249 |
| R-free | 0.26900 |
| Structure solution method | fourier difference |
| Starting model (for MR) | 1r0k 1-deoxy-D-xylulose 5-phosphate reductoisomerase |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.600 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.630 | 2.850 |
| High resolution limit [Å] | 2.690 | 2.700 |
| Rmerge | 0.376 | |
| Number of reflections | 43474 | |
| <I/σ(I)> | 10.1 | 3.1 |
| Completeness [%] | 99.6 | 99.6 |
| Redundancy | 3.1 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | PEG4000, Na citrate, Ammonium acetate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
