1QZ7
Beta-catenin binding domain of Axin in complex with beta-catenin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2002-12-05 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.0000 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 85.141, 75.041, 101.398 |
Unit cell angles | 90.00, 97.47, 90.00 |
Refinement procedure
Resolution | 42.210 - 2.200 |
R-factor | 0.221 |
Rwork | 0.221 |
R-free | 0.25600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1i7x |
RMSD bond length | 0.006 |
RMSD bond angle | 18.400 * |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.180 |
High resolution limit [Å] | 2.200 * | 2.100 |
Rmerge | 0.072 | 0.529 * |
Number of reflections | 37253 | |
<I/σ(I)> | 17.8 | 1.8 |
Completeness [%] | 99.8 | 99.9 * |
Redundancy | 3.7 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 * | 20 * | PEG 6000, sodium citrate, isopropanol, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 2 (mg/ml) | |
2 | 1 | drop | Tris | 20 (mM) | pH8.5 |
3 | 1 | drop | 0.15 (M) | ||
4 | 1 | drop | dithiothreitol | 2 (mM) | |
5 | 1 | reservoir | sodium citrate | 0.1 (M) | pH5.6 |
6 | 1 | reservoir | isopropanol | 15 (%(v/v)) | |
7 | 1 | reservoir | PEG4000-6000 | 7 (%(w/v)) | |
8 | 1 | reservoir | dithiothreitol | 5 (mM) |