1QX7
Crystal structure of apoCaM bound to the gating domain of small conductance Ca2+-activated potassium channel
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL1-5 |
Synchrotron site | SSRL |
Beamline | BL1-5 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-05-14 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.05 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 79.930, 79.930, 220.800 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 75.160 - 3.090 |
R-factor | 0.286 |
Rwork | 0.286 |
R-free | 0.30800 |
Structure solution method | MR combined with MAD |
Starting model (for MR) | 1qx5 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.100 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | CNS |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 75.160 | 3.510 |
High resolution limit [Å] | 3.090 | 3.090 |
Completeness [%] | 99.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | Citrate, NaCl, Hepes, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |