1QX7
Crystal structure of apoCaM bound to the gating domain of small conductance Ca2+-activated potassium channel
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL1-5 |
| Synchrotron site | SSRL |
| Beamline | BL1-5 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-05-14 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.05 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 79.930, 79.930, 220.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 75.160 - 3.090 |
| R-factor | 0.286 |
| Rwork | 0.286 |
| R-free | 0.30800 |
| Structure solution method | MR combined with MAD |
| Starting model (for MR) | 1qx5 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.100 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | CNS |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 75.160 | 3.510 |
| High resolution limit [Å] | 3.090 | 3.090 |
| Completeness [%] | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | Citrate, NaCl, Hepes, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
