1QX7

Crystal structure of apoCaM bound to the gating domain of small conductance Ca2+-activated potassium channel

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL1-5
Synchrotron site	SSRL
Beamline	BL1-5
Temperature [K]	100
Detector technology	CCD
Collection date	2002-05-14
Detector	ADSC QUANTUM 4
Wavelength(s)	1.05
Spacegroup name	P 41 21 2
Unit cell lengths	79.930, 79.930, 220.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	75.160 - 3.090
R-factor	0.286
Rwork	0.286
R-free	0.30800
Structure solution method	MR combined with MAD
Starting model (for MR)	1qx5
RMSD bond length	0.008
RMSD bond angle	1.100
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	CNS
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	75.160	3.510
High resolution limit [Å]	3.090	3.090
Completeness [%]		99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	298	Citrate, NaCl, Hepes, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K