1QX5
Crystal structure of apoCalmodulin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-02-12 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.989 |
| Spacegroup name | P 31 |
| Unit cell lengths | 146.000, 146.000, 78.000 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 66.390 - 2.540 |
| R-factor | 0.227 |
| Rwork | 0.227 |
| R-free | 0.27700 |
| Structure solution method | MIR |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.600 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | CNS |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.400 | 2.700 |
| High resolution limit [Å] | 2.540 | 2.540 |
| Number of reflections | 61810 | |
| <I/σ(I)> | 10.2 | |
| Completeness [%] | 98.5 | 98.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | Citrate, 150 mM NaCl, Hepes , pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
