1QX4
Structrue of S127P mutant of cytochrome b5 reductase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X12B |
Synchrotron site | NSLS |
Beamline | X12B |
Temperature [K] | 99 |
Detector technology | CCD |
Collection date | 2001-05-15 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.98 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 77.236, 48.004, 77.247 |
Unit cell angles | 90.00, 107.69, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.800 |
R-factor | 0.207 |
Rwork | 0.207 |
R-free | 0.24100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1IB0 without FAD molecule |
RMSD bond length | 0.013 |
RMSD bond angle | 1.400 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 30.000 |
High resolution limit [Å] | 1.800 |
Rmerge | 0.064 |
Number of reflections | 48325 |
Completeness [%] | 96.0 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | glycerol, ammonium sulfate, tris buffer, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K |