1QW9

Crystal structure of a family 51 alpha-L-arabinofuranosidase in complex with 4-nitrophenyl-Ara

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7B
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7B
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2002-09-16
Detector	MARRESEARCH
Wavelength(s)	0.84590
Spacegroup name	H 3
Unit cell lengths	179.311, 179.311, 100.397
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 1.200
R-factor	0.173
Rwork	0.160
R-free	0.17900
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014 *
RMSD bond angle	1563.000 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.240
High resolution limit [Å]	1.200	1.200
Rmerge	0.049 *	0.383 *
Number of reflections	370260 *
Completeness [%]	99.1	97.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	18 % PEG 3350, 0.2 M NH4F, 5 % 2-propanol, 0.1 M Tris/HCl, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG3350	23 (%(w/v))
2	1	reservoir		0.2 (M)
3	1	reservoir	2-propanol	5 (%(v/v))
4	1	reservoir	Tris-HCl	0.1 (M)	pH8.0