1QSM

Histone Acetyltransferase HPA2 from Saccharomyces Cerevisiae in Complex with Acetyl Coenzyme A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	CCD
Collection date	1998-01-01
Detector	BRANDEIS
Spacegroup name	C 2 2 21
Unit cell lengths	145.600, 184.100, 70.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 * - 2.400
R-factor	0.168
Rwork	0.168
R-free	0.24400
RMSD bond length	0.007
RMSD bond angle	1.100
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	X-PLOR (3.843)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.530
High resolution limit [Å]	2.400	2.400
Rmerge	0.045	0.113
Total number of observations	456328 *
Number of reflections	37150 *
<I/σ(I)>	21
Completeness [%]	100.0	99.7
Redundancy	6.5	8.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.9 *	4 *	PEG 4000, MES, CALCIUM ACETATE, pH 6.00, VAPOR DIFFUSION, HANGING DROP, temperature 277.00K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	PEG4000	8 (%(w/v))
3	1	reservoir	MES	0.1 (M)
4	1	reservoir	calcium acetate	15 (M)