1QSM
Histone Acetyltransferase HPA2 from Saccharomyces Cerevisiae in Complex with Acetyl Coenzyme A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1998-01-01 |
Detector | BRANDEIS |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 145.600, 184.100, 70.000 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 * - 2.400 |
R-factor | 0.168 |
Rwork | 0.168 |
R-free | 0.24400 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.100 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | X-PLOR (3.843) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.530 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.045 | 0.113 |
Total number of observations | 456328 * | |
Number of reflections | 37150 * | |
<I/σ(I)> | 21 | |
Completeness [%] | 100.0 | 99.7 |
Redundancy | 6.5 | 8.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.9 * | 4 * | PEG 4000, MES, CALCIUM ACETATE, pH 6.00, VAPOR DIFFUSION, HANGING DROP, temperature 277.00K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | reservoir | PEG4000 | 8 (%(w/v)) | |
3 | 1 | reservoir | MES | 0.1 (M) | |
4 | 1 | reservoir | calcium acetate | 15 (M) |