1QRZ
CATALYTIC DOMAIN OF PLASMINOGEN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12B |
| Synchrotron site | NSLS |
| Beamline | X12B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1998-10-14 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 81.658, 73.575, 81.363 |
| Unit cell angles | 90.00, 108.73, 90.00 |
Refinement procedure
| Resolution | 35.000 - 2.000 |
| R-factor | 0.235 |
| Rwork | 0.235 |
| R-free | 0.29400 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.900 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (0.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.165 | 0.631 |
| Total number of observations | 199470 * | |
| Number of reflections | 63217 | |
| <I/σ(I)> | 11 | |
| Completeness [%] | 99.3 | 98 |
| Redundancy | 6.9 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 * | 277 | PEG 4000, ISOPROPANOL, CITRATE, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | drop | benzamidine | 50 (mM) | |
| 3 | 1 | drop | sodium cacodylate | 100 (mM) | |
| 4 | 1 | reservoir | 2-propanol | 3 (%) | |
| 5 | 1 | reservoir | PEG4000 | 3 (%) | |
| 6 | 1 | reservoir | citrate | 20 (mM) |
