1QRQ
STRUCTURE OF A VOLTAGE-DEPENDENT K+ CHANNEL BETA SUBUNIT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-09-19 |
| Detector | RIGAKU RAXIS |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 156.746, 114.346, 93.050 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.800 |
| R-factor | 0.236 |
| Rwork | 0.236 |
| R-free | 0.24200 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.880 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.074 | 0.394 |
| Total number of observations | 814316 * | |
| Number of reflections | 40961 | |
| <I/σ(I)> | 15.8 | |
| Completeness [%] | 97.7 | 98.1 |
| Redundancy | 19.4 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 | pH 7.50 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 27 (mg/ml) | |
| 2 | 1 | drop | n-octyl-beta-D-glucopyranoside | 4.5 (mM) | |
| 3 | 1 | reservoir | HEPES | 100 (mM) | pH7.5 |
| 4 | 1 | reservoir | mPEG2000 | 26 (%(w/v)) | |
| 5 | 1 | reservoir | ammonium sulfate | 400 (mM) |
