1QPG
3-PHOSPHOGLYCERATE KINASE, MUTATION R65Q
Experimental procedure
| Detector technology | AREA DETECTOR |
| Collection date | 1991-09-30 |
| Detector | SIEMENS |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 96.000, 70.100, 82.300 |
| Unit cell angles | 90.00, 122.20, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.400 |
| R-factor | 0.185 |
| Rwork | 0.185 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 21.670 * |
| Data reduction software | XENGEN |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 40.700 |
| High resolution limit [Å] | 2.430 |
| Rmerge | 0.076 |
| Total number of observations | 100828 * |
| Number of reflections | 16281 |
| Completeness [%] | 92.4 |
| Redundancy | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | R65Q PGK | 18 (mg/ml) | |
| 2 | 1 | drop | 5'-adenylylimidodiphosphate | 10 (mM) | |
| 3 | 1 | drop | tetralithium salt (AMP-PNP) | ||
| 4 | 1 | drop | D-(-)-3-phosphoglycerate | 10 (mM) | |
| 5 | 1 | drop | disodium salt (3-PG) | ||
| 6 | 1 | reservoir | PEG8000 | 21 (%) | |
| 7 | 1 | reservoir | magnesium acetate | 20 (mM) | |
| 8 | 1 | reservoir | 0.02 (%) | ||
| 9 | 1 | reservoir | HEPES | 50 (mM) |
