1QO8
The structure of the open conformation of a flavocytochrome c3 fumarate reductase
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | BW7B |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1999-04-15 |
Detector | MARRESEARCH |
Wavelength(s) | 0.8469, 1.033, 1.739, 1.741 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 71.770, 109.690, 227.320 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.150 |
R-factor | 0.225 * |
Rwork | 0.225 |
R-free | 0.28100 |
Structure solution method | MAD |
RMSD bond length | 0.015 |
RMSD bond angle | 0.013 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.190 |
High resolution limit [Å] | 2.150 | 2.150 |
Rmerge | 0.038 * | 0.274 * |
Total number of observations | 1428311 * | |
Number of reflections | 97968 | |
Completeness [%] | 93.4 * | 91.4 |
Redundancy | 14.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.5 * | Bamford, V., (1999) Acta Crystallogr.,Sect., D55, 1222. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10-15 (mg/ml) | |
2 | 1 | drop | sodium HEPES | 50 (mM) | |
3 | 1 | drop | 100 (mM) | ||
4 | 1 | reservoir | PEG10000 | 6-15 (%(w/v)) | |
5 | 1 | reservoir | sodium MES |