1QNF

STRUCTURE OF PHOTOLYASE

Experimental procedure

Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6A
Synchrotron site	Photon Factory
Beamline	BL-6A
Temperature [K]	293
Detector technology	IMAGE PLATE
Spacegroup name	P 43 21 2
Unit cell lengths	91.170, 91.170, 135.540
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 1.800
R-factor	0.197
Rwork	0.197
R-free	0.23900
Structure solution method	MIR
RMSD bond length	0.009
RMSD bond angle	22.980 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000
High resolution limit [Å]	1.800	1.800
Rmerge	0.076
Total number of observations	246336 *
Number of reflections	44414 *
Completeness [%]	83.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7	4 *	20%(W/V) AMMONIUM SULFATE, 10MM POTASSIUM PHOSPHATE (PH7.0)

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	30 (mg/ml)
2	1	drop	potassium phosphate	10 (mM)
3	1	drop	2-mercaptoethanol	2 (mM)
4	1	drop	ammonium sulfate	8-10 (%(w/v))
5	1	reservoir	ammonium sulfate	22 (%(w/v))