1QMI
Crystal structure of RNA 3'-terminal phosphate cyclase, an ubiquitous enzyme with unusual topology
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X9B |
| Synchrotron site | NSLS |
| Beamline | X9B |
| Temperature [K] | 105 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-01-28 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 101.800, 126.600, 128.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.800 |
| R-factor | 0.244 |
| Rwork | 0.244 |
| R-free | 0.33100 |
| Structure solution method | MAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 2.100 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.045 | 0.198 |
| Total number of observations | 154068 * | |
| Number of reflections | 41596 | |
| <I/σ(I)> | 17.8 | 7.5 |
| Completeness [%] | 96.9 | 91.6 |
| Redundancy | 3.9 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5.5 | 20 * | THE PROTEIN WAS CRYSTALLIZED FROM 13-15% MPEG2000, 200 MM NA-CITRATE PH 4.0, 200 MM TRIS/HCL PH 8.0, 2 MM DTT. PROTEIN CONCENTRATION WAS CA. 15 MG/ML. |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | reservoir | mPEG2000 | 13-15 (%) | |
| 3 | 1 | reservoir | Na citrate | 200 (mM) | |
| 4 | 1 | reservoir | Tris-HCl | 200 (mM) | |
| 5 | 1 | reservoir | dithiothreitol | 2 (mM) |
