1QMI

Crystal structure of RNA 3'-terminal phosphate cyclase, an ubiquitous enzyme with unusual topology

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X9B
Synchrotron site	NSLS
Beamline	X9B
Temperature [K]	105
Detector technology	IMAGE PLATE
Collection date	1998-01-28
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	101.800, 126.600, 128.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.800
R-factor	0.244
Rwork	0.244
R-free	0.33100
Structure solution method	MAD
RMSD bond length	0.018
RMSD bond angle	2.100
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELX
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.045	0.198
Total number of observations	154068 *
Number of reflections	41596
<I/σ(I)>	17.8	7.5
Completeness [%]	96.9	91.6
Redundancy	3.9	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.5	20 *	THE PROTEIN WAS CRYSTALLIZED FROM 13-15% MPEG2000, 200 MM NA-CITRATE PH 4.0, 200 MM TRIS/HCL PH 8.0, 2 MM DTT. PROTEIN CONCENTRATION WAS CA. 15 MG/ML.

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	reservoir	mPEG2000	13-15 (%)
3	1	reservoir	Na citrate	200 (mM)
4	1	reservoir	Tris-HCl	200 (mM)
5	1	reservoir	dithiothreitol	2 (mM)