1QK1
CRYSTAL STRUCTURE OF HUMAN UBIQUITOUS MITOCHONDRIAL CREATINE KINASE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ELLIOTT GX-18 |
| Temperature [K] | 277 |
| Detector technology | AREA DETECTOR |
| Collection date | 1998-12-15 |
| Detector | MULTIWIRE SIEMENS |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 91.811, 125.868, 212.074 |
| Unit cell angles | 90.00, 96.71, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.700 |
| R-factor | 0.195 |
| Rwork | 0.195 |
| R-free | 0.21900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1crk SARCOMERIC MITOCHONDRIAL CREATINE KINASE |
| RMSD bond length | 0.010 |
| RMSD bond angle | 23.600 * |
| Data reduction software | XDS (V. 99) |
| Data scaling software | XDS (V. 99) |
| Phasing software | CNS (0.5) |
| Refinement software | CNS (0.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.092 | 0.313 |
| Total number of observations | 616668 * | |
| Number of reflections | 130945 | 13429 * |
| <I/σ(I)> | 11.1 | 2.7 |
| Completeness [%] | 99.4 | 99.1 |
| Redundancy | 4.7 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 6.75 | 4 * | 0.025M NA-PHOSPHATE, 3 MM DTT, PH 6.75, 5 MG/ML PROTEIN |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | sodium phosphate | 25 (mM) | |
| 2 | 1 | 1 | beta-mercaptoethanol | 2 (mM) | |
| 3 | 1 | 1 | EDTA | 0.2 (mM) | |
| 4 | 1 | 1 | protein | 5 (mg/ml) |
