1QJS
mammalian blood serum haemopexin glycosylated-native protein and in complex with its ligand haem
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-06-15 |
| Detector | RIGAKU IMAGE PLATE |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 103.900, 69.900, 151.810 |
| Unit cell angles | 90.00, 108.16, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.900 |
| R-factor | 0.251 |
| Rwork | 0.255 |
| R-free | 0.31200 |
| Structure solution method | SIR |
| Starting model (for MR) | 1HXN AND 1FBL |
| RMSD bond length | 0.013 |
| RMSD bond angle | 0.041 |
| Data reduction software | DENZO |
| Data scaling software | Agrovata |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.050 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.064 | 0.251 |
| Number of reflections | 20915 | |
| <I/σ(I)> | 6.2 | 2.2 |
| Completeness [%] | 97.5 | 87.5 |
| Redundancy | 2.2 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.9 | 4 * | HANGING DROP, 4 DEGREES CENTIGRADE. RESEVOIR SOLUTION: 15-22% PEG 6000, 0.1-0.2 M TRIS HCL PH 7.9, 0.05-0.1 M EDTA, 0.05-0.2 NACL, PROTEIN COMPLEX SOLUTION: 65 MG/ML IN 0.01 M TRIS PH 7.9, 0.01 M NACL, 0.1 M EDTA |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 65 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 0.01 (M) | |
| 3 | 1 | drop | EDTA | 0.01 (M) | |
| 4 | 1 | drop | 0.01 (M) | ||
| 5 | 1 | reservoir | PEG6000 | 15-22 (%(w/v)) | |
| 6 | 1 | reservoir | Tris-HCl | 0.1-0.2 (M) | |
| 7 | 1 | reservoir | EDTA | 0.05-0.1 (M) | |
| 8 | 1 | reservoir | 0.05-0.2 (M) |
