1QJ0
HUMAN INSULIN HEXAMERS WITH CHAIN B HIS MUTATED TO TYR
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH2R |
Temperature [K] | 289 |
Detector technology | IMAGE PLATE |
Detector | RIGAKU IMAGE PLATE |
Spacegroup name | H 3 |
Unit cell lengths | 80.940, 80.940, 37.630 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 25.700 - 2.400 |
Rwork | 0.199 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | T3R3 (NATIVE) INSULIN |
RMSD bond length | 0.021 |
RMSD bond angle | 0.054 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Refinement software | PROLSQ |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.700 | 2.100 |
High resolution limit [Å] | 2.400 | 2.000 |
Rmerge | 0.046 | 0.132 |
Number of reflections | 3477 | |
Completeness [%] | 97.0 | 92 |
Redundancy | 2.5 | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Batch method * | 6.8 | CRYSTALLISATION IN BATCH: 10 MG B5 TYR INSULIN DISSOLVED IN 2 ML 0.02M HCL. TO THIS ADDED 0.05 ML 0.15M ZINC ACETATE, 1.0 ML 0.2 M TRI-SODIUM CITRATE, 1.0 ML ACETONE. PH ADJUSTED TO 6.4-7.1 . |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | insulin | 10 (mg/ml) | |
2 | 1 | 1 | 0.02 (M) | 2 ml | |
3 | 1 | 1 | zinc acetate | 0.15 (M) | 0.05 ml |
4 | 1 | 1 | trisodium citrate | 0.2 (M) | 1 ml |
5 | 1 | 1 | acetone | 1 ml |