1QG4

CANINE GDP-RAN F72Y MUTANT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 5.2R
Synchrotron site	ELETTRA
Beamline	5.2R
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1998-01-29
Detector	MAR scanner 345 mm plate
Spacegroup name	P 1 21 1
Unit cell lengths	60.520, 60.170, 61.440
Unit cell angles	90.00, 101.77, 90.00

Refinement procedure

Resolution	6.000 - 2.500
R-factor	0.174 *
Rwork	0.174
R-free	0.23700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	EBI-1279
RMSD bond length	0.011 *
RMSD bond angle	2.000 *
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.174	2.640
High resolution limit [Å]	2.500	2.500
Rmerge	0.052	0.158
Number of reflections	14415
<I/σ(I)>	9.7	4.7
Completeness [%]	96.0	96.3
Redundancy	2	2.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	8 *	18 *	pH 7.20

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	PEG1000	20-36 (%(v/v))
3	1	reservoir		20 (mM)
4	1	reservoir	Tris-HCl	50 (mM)