1QE6
INTERLEUKIN-8 WITH AN ADDED DISULFIDE BETWEEN RESIDUES 5 AND 33 (L5C/H33C)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1996-10-15 |
| Detector | PRINCETON 1K |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.540, 71.770, 57.860 |
| Unit cell angles | 90.00, 90.46, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.350 |
| R-factor | 0.206 * |
| Rwork | 0.208 |
| R-free | 0.27700 * |
| RMSD bond length | 0.006 * |
| RMSD bond angle | 1.000 |
| Data reduction software | MCCDATA |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.430 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.088 | 0.113 |
| Number of reflections | 12188 | |
| <I/σ(I)> | 13 | |
| Completeness [%] | 95.0 | 86 |
| Redundancy | 4.3 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 20 * | NACL, AMMONIUM SULFATE, PEG 8000, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 19K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 4.2 (mg/ml) | |
| 2 | 1 | drop | 100 (mM) | ||
| 3 | 1 | drop | MES | 4 (mM) | |
| 4 | 1 | drop | ammonium sulfate | 60 (mM) | |
| 5 | 1 | drop | PEG8000 | 18 (%(w/v)) | |
| 6 | 1 | reservoir | ammonium sulfate | 100 (mM) | |
| 7 | 1 | reservoir | PEG8000 | 30 (%(w/v)) |
