1QBK

STRUCTURE OF THE KARYOPHERIN BETA2-RAN GPPNHP NUCLEAR TRANSPORT COMPLEX

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	CCD
Collection date	1998-07-09
Detector	CUSTOM-MADE
Spacegroup name	P 32 2 1
Unit cell lengths	133.110, 133.110, 138.410
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	500.000 - 3.000
R-factor	0.267
Rwork	0.267
R-free	0.31500
RMSD bond length	0.090
RMSD bond angle	1.400
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	500.000
High resolution limit [Å]	3.000
Rmerge	0.095
Total number of observations	333715 *
Number of reflections	54577 *
<I/σ(I)>	9.4
Completeness [%]	99.4
Redundancy	6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	HEPES, MGCL2, PEG3350, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 20K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	HEPES	100 (mM)
2	1	reservoir	PEG3350	28 (%)
3	1	reservoir		0.2 (M)