1QBG

CRYSTAL STRUCTURE OF HUMAN DT-DIAPHORASE (NAD(P)H OXIDOREDUCTASE)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	1996-10-15
Detector	RIGAKU RAXIS II
Spacegroup name	P 1
Unit cell lengths	55.880, 57.490, 98.770
Unit cell angles	77.10, 76.20, 86.90

Refinement procedure

Resolution	40.000 - 2.300
R-factor	0.232
R-free	0.28000
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.400
High resolution limit [Å]	2.300	2.300
Rmerge	0.132	0.600
Total number of observations	193332 *
Number of reflections	43746
<I/σ(I)>	4.98
Completeness [%]	81.5 *	70

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	18 *	Sitting drop, PEG4000, CYMAL-3 detergent, sodium phosphate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	9.7 (mg/ml)
2	1	drop	sodium phosphate	100 (mM)
3	1	reservoir	PEG4000	25 (%(w/v))