1QAS
1-PHOSPHATIDYLINOSITOL-4,5-BISPHOSPHATE PHOSPHODIESTERASE DELTA 1
Experimental procedure
Source type | ROTATING ANODE |
Source details | RIGAKU RUH2R |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1995-10 |
Detector | RIGAKU |
Spacegroup name | P 1 |
Unit cell lengths | 61.100, 75.400, 86.900 |
Unit cell angles | 66.90, 85.40, 89.80 |
Refinement procedure
Resolution | 6.000 - 2.400 |
R-factor | 0.212 |
Rwork | 0.212 |
R-free | 0.28600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | CUBIC PHOSPHOLIPASE C |
RMSD bond length | 0.011 |
RMSD bond angle | 24.900 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR |
Refinement software | X-PLOR |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 60.000 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.063 | 0.296 |
Number of reflections | 47893 | |
<I/σ(I)> | 10.9 | 2.1 |
Completeness [%] | 86.4 | 44.5 |
Redundancy | 1.76 | 1.42 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 6.5 | used to seeding, Grobler, J.A., (1996) Protein Sci., 5, 680. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | NaMes | 0.1 (M) | |
2 | 1 | reservoir | 0.2 (M) | ||
3 | 1 | reservoir | glycerol | 20 (%) | |
4 | 1 | reservoir | PEG8000 | 6-8 (%) |