1Q8H
Crystal structure of porcine osteocalcin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.7 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 51.491, 51.491, 35.389 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.720 - 2.000 |
| R-factor | 0.255 |
| Rwork | 0.255 |
| R-free | 0.28300 |
| Structure solution method | ISAS |
| RMSD bond length | 0.006 |
| RMSD bond angle | 20.500 * |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.067 * | 0.219 * |
| Number of reflections | 6230 | |
| Completeness [%] | 95.9 * | 93.8 * |
| Redundancy | 23 * | 5 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 298 | PEG 4000, Calcium Chloride, HEPES, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | HEPES | 100 (mM) | pH7.4 |
| 2 | 1 | drop | protein | 10 (mg/ml) | |
| 3 | 1 | reservoir | HEPES | 0.1 (M) | |
| 4 | 1 | reservoir | 10 (mM) | ||
| 5 | 1 | reservoir | PEG4000 | 10 (%(w/v)) | pH7.4 |
