1Q8H
Crystal structure of porcine osteocalcin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MARRESEARCH |
Wavelength(s) | 1.7 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 51.491, 51.491, 35.389 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 27.720 - 2.000 |
R-factor | 0.255 |
Rwork | 0.255 |
R-free | 0.28300 |
Structure solution method | ISAS |
RMSD bond length | 0.006 |
RMSD bond angle | 20.500 * |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.067 * | 0.219 * |
Number of reflections | 6230 | |
Completeness [%] | 95.9 * | 93.8 * |
Redundancy | 23 * | 5 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 298 | PEG 4000, Calcium Chloride, HEPES, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | HEPES | 100 (mM) | pH7.4 |
2 | 1 | drop | protein | 10 (mg/ml) | |
3 | 1 | reservoir | HEPES | 0.1 (M) | |
4 | 1 | reservoir | 10 (mM) | ||
5 | 1 | reservoir | PEG4000 | 10 (%(w/v)) | pH7.4 |