1Q8D

The crystal structure of GDNF family co-receptor alpha 1 domain 3

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7A
Synchrotron site	EMBL/DESY, Hamburg
Beamline	BW7A
Temperature [K]	100
Detector technology	CCD
Collection date	2002-11-30
Detector	MARRESEARCH
Wavelength(s)	0.9635
Spacegroup name	P 61
Unit cell lengths	61.334, 61.334, 65.154
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.190 - 1.800
R-factor	0.193
Rwork	0.193
R-free	0.20800
Structure solution method	MAD
RMSD bond length	0.004
RMSD bond angle	1.000
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS (1.1)
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.860
High resolution limit [Å]	1.800	1.800
Number of reflections	12986
<I/σ(I)>	41.2	9.4
Completeness [%]	100.0	99.8
Redundancy	10.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	277	MPD, MES, magnesium chloride, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K