1Q78
Crystal structure of poly(A) polymerase in complex with 3'-dATP and magnesium chloride
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X12C |
Synchrotron site | NSLS |
Beamline | X12C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-08-03 |
Detector | CUSTOM-MADE |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 57.520, 63.810, 180.420 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.760 - 2.800 |
R-factor | 0.204 |
Rwork | 0.204 |
R-free | 0.25500 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.006 |
RMSD bond angle | 1.300 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.970 |
High resolution limit [Å] | 2.800 | 2.800 |
Number of reflections | 15770 | |
Completeness [%] | 92.8 | 64.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 297 | PEG8000, Ammonium sulfate, MES buffer, calcium chloride, magnesium chloride, pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 297K |